Publications

Below you will find a list of some publications where the Cellevate 3D™ NanoMatrix has been used. Want to see your publication on our list? Send your work to info@cellevate.com and we will get back to you shortly!

Scientific Reports Published March 2021

Identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds

The appreciation that cell interactions in tissues is dependent on their three dimensional (3D) distribution has stimulated the development of 3D cell culture models. We constructed an artificial 3D tumour by culturing human breast cancer JIMT-1 cells and human dermal fibroblasts (HDFs) in a 3D network of electrospun polycaprolactone fibres. Here, we investigate ECM components produced by the cells in the artificial 3D tumour, which is an important step in validating the model.

Toxicology in Vitro Published October 2019

Unique animal friendly 3D culturing of human cancer and normal cells

Two-dimensional cell culturing has proven inadequate as a reliable preclinical tumour model due to many inherent limitations. Hence, novel three-dimensional cell culture models are needed, which in many aspects can mimic a native tumour with 3D extracellular matrix. Here, we present a 3D electrospun polycaprolactone (PCL) mesh mimicking the collagen network of tissue.

Animal Models of Neurotrauma Published July 2019

Traumatic Peripheral Nerve Injuries: Experimental Models for Repair and Reconstruction

Peripheral nerve injuries are difficult to treat, and the clinical outcome after surgical repair and reconstruction is still insufficient, particularly concerning recovery of sensory function. Rat models, where the sciatic nerve has essentially a similar size as a human digital nerve, are widely used to evaluate nerve regeneration with the inherent advantages and disadvantages of the experimental models.

Scientific Reports, volume 8 Published November 2018

Electrospun nerve guide conduits have the potential to bridge peripheral nerve injuries in vivo

Electrospinning can be used to mimic the architecture of an acellular nerve graft, combining microfibers for guidance, and pores for cellular infiltration. We made electrospun nerve guides, from polycaprolactone (PCL) or poly-L-lactic acid (PLLA), with aligned fibers along the insides of the channels and random fibers around them.

MEA Meeting 2018 | 11th International Meeting on Substrate Integrated Microelectrode Arrays.

Assessing electrical activity of human neuronal cell culture networks grown on 3D fibrous scaffolds using different techniques: A comparative study

Two-dimensional cell culturing has proven inadequate as a reliable preclinical tumour model due to many inherent limitations. Hence, novel three-dimensional cell culture models are needed, which in many aspects can mimic a native tumour with 3D extracellular matrix. Here, we present a 3D electrospun polycaprolactone (PCL) mesh mimicking the collagen network of tissue..

J. Nanomedicine: Nanotechnology, Biology, and Medicine Published January 2017

Three-dimensional functional human neuronal networks in uncompressed low-density electrospun fiber scaffolds

We demonstrate an artificial three-dimensional (3D) electrical active human neuronal network system, by the growth of brain neural progenitors in highly porous low density electrospun poly-ε-caprolactone (PCL) fiber scaffolds…

J. Biomaterials and Nanobiotechnology Published January 2017

Tailor-Made Electrospun Culture Scaffolds Control Human Neural Progenitor Cell Behavior — Studies on Cellular Migration and Phenotypic Differentiation

We explored how basic properties of neural cells, nuclei polarization, phenotypic differentiation and distribution/migration, were affected by the culture at poly-L-lactic acid (PLLA) fibrous scaffolds, using a multipotent mitogen-expanded human neural progenitor cell (HNPC) line.

J. PLoS ONE Published January 2017

Accelerated Wound Closure – Differently Organized Nanofibers Affect Cell Migration and Hence the Closure of Artificial Wounds in a Cell Based In Vitro Model

Here we explored the influence of nanofiber alignment on fibroblast behavior in a novel in vitro wound model. The model included electrospun poly-ε-caprolactone scaffolds with different nanofiber orientation.

Molecular and Cellular Neuroscience Published September 2016

Exploration of physical and chemical cues on retinal cell fate

Identification of the key components in the physical and chemical milieu directing donor cells into a desired phenotype is a requirement in the investigation of bioscaffolds for the advancement of cell-based therapies for retinal neurodegeneration.

J. Cellular Physiology Published January 2015

3D Cell Culture Systems: Advantages and Applications

Here, we review the three dimensional way of culturing cells, their advantages, the scaffolds and matrices currently available, and the applications of such cultures in major areas of life sciences.