Life Science Research
Since Cellevate was founded in 2014 we have worked with our collaborators in most fields of life-science research, primarily focusing on the areas of oncology, immunology and neurodegenerative diseases. Cellevat3d™ products have already been used and generated results across a range of cell culture applications. Some of the cells used are outlined below:
- Chinese Hamster Ovary Cells (CHO)
- Human Embryonic Kidney Cells (HEK293AD, HEK293T)
- Human Caucasian Carcinoma Cells (A549)
- Human Colon Carcinoma Cells (HCT116)
- Mesenchymal Stem Cells (MCS)
- Induced Pluripiotent Stem Cells (iPSC)
- Hematopoietic Stem Cells (HSC)
- Human Neural Progenitor Cells (HNPC)
- Retinal Post-Natal Cells (RPNC)
- Peripheral Mononuclear Cells (PBMC)
- Human Adipose Tissue-Derived Stem Cells (hASC)
- Fibroblasts (Murine, Human-Dermal, cancer associated)
- Microglia (BV2)
- Breast Epithelial Cells (MCF10A)
- Breast Cancer Cells (JIMT-1)
- Circulating Tumor Cells (CTC)
- Natural Killer Cells (NK)
- Human Neuroblastoma Cells (SH-SY5Y)
- Pancreatic Cancer Cells (MIA PaCa-2)
- Pancreatic Ductal Adenocarcinoma Cells (PDAC)
On this page you will find information on the available, already validated models and assays we have developed.
Breast Cancer
In a collaboration with Lund University we’ve developed a scaffold for co-culture of breast cancer research.
We have successfully demonstrated that different cell types, malignant as well as normal thrive in the 3D fibre unit. We are presently exploring this model for co-culturing of cancer cells and different types of stromal cells. We anticipate this model can be used to create a 3D tumour ex vivo platform that can be used for screening of therapeutic compounds, which will reduce the number of animals in cancer research.
Validated readouts:
- Cell morphology
- Cell infiltration
- Cell proliferation
Analytical methods:
- Confocal microscopy
- AlamarBlue® assay
- Cryosectioning
- Scanning electron microscopy (SEM)
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Pancreatic Cancer
In a collaboration with Swedish CRO we’ve developed a scaffold for stable, long-term co-culture of pancreatic cancer- and stromal cells.
Their model for pancreatic cancer co-cultures, a traditional submerged 2D system had performance issues. Limited space meant contact inhibition was problematic and the system became over confluent around 4 DIV, which was not enough time to develop a disease relevant culture.
The model is currently being used to further investigate drug sensitivity and cytotoxicity.
Validated readouts:
- Cell morphology
- Cell infiltration
- Cell proliferation
- Biomarker expression
- Dose-response
Analytical methods:
- Confocal microscopy
- AlamarBlue® assay
- ELISA
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Neurodegenerative Diseases:
iPSC culture for psychiatric disease
In a collaboration with European Pharmaceutical company we’ve developed a scaffold for culture of iPSCs for studying psychiatric disease.
Neurons and astrocytes matured faster in 3D culture compared to conventional 2D culture and the co-cultures could be maintained for several weeks without affecting cell viability. First stainings indicate that neurons develop a very dense neuronal network and surrounding astrocytes are in close interaction with them.
More functional readouts are currently being validated for the model.
Validated readouts:
- Cell morphology
- Cell infiltration
Analytical methods:
- Confocal microscopy
- Light-sheet microscopy
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Neurodegenerative Diseases:
HNPC culture for basic research
In a collaboration with Lund University we’ve developed a scaffold for suitable for both human neuronal progenitor cells (HNPCs) and retinal cells.
Successfully, we here demonstrate a functional and true 3D neuronal network after seeding of human neural progenitors into uncompressed highly porous electrospun PCL fiber scaffold. Notably, glial cells were found integrated in 3D in an in vivo mimicking way — both in the scaffolds as well as with neighboring neurons. Further exploration of such uncompressed electrospun scaffolds is highly motivated, with different neural tool cells for both basic neurobiology studies and in neural engineering approaches where they can be investigated for support and axonal guidance upon implantation into the nervous system.
Validated readouts:
- Cell proliferation
- Cell morphology
- Cell migration
- Protein expression
- Neuronal activity
- RNA expression
Analytical methods:
- AlamarBlue® assay
- Scanning electron microscopy (SEM)
- Confocal Microscopy
- Fluorescence microscopy
- Western Blot
- Electrophysiology
- RT-qPCR
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Immunology assay:
Ex vivo activation of human primary cells
The ex-vivo activation model is an excellent tool for initial evaluation of effects on the immune system, ranking of compounds or mechanism of action studies. Human peripheral blood mononuclear cells (PBMCs) comprising lymphocytes (T cells, B cells, and NK cells), dendritic cells, and monocytes are activated under different conditions ex-vivo and immunological effects
evaluated. In this experimental setup, we activate inflammatory cells in 3D cultures resembling the local inflammatory
response in-vivo. The assay can be adapted to target cells and inflammatory markers of interest.
Human PBMCs are activated in a 3D system with LPS, anti-CD3/anti-CD28 or ConA (depending on cell type and pathway of interest) in presence of reference drugs or test item for 24 hours and analysed for cellular marker expression using flow cytometry. The supernatant is analysed for cytokine profile using luminex technology. In addition to the standard readouts, there is an option to add on analysis of proliferation and viability or other project specific markers.
CELLULAR COMPOSITION (FLOW CYTOMETRY)
PBMCs are activated with anti-CD3/CD28 (T cells) or LPS (innate immune system) in presence of reference drug e.g. Dexamethasone and test item for 24 hours. Cells analysed for expression of cellular markers and cellular composition using flow cytometry.
CYTOKINE PROFILE (LUMINEX)
Supernatant is investigated for standard cytokines using Luminex and a standard inflammation panel or project specific custom adapted panel.
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Immunology assay:
Ex vivo activation of mouse splenocytes
The ex-vivo activation model is an excellent tool for initial evaluation of effects on the immune system, ranking of compounds or mechanism of action studies. Mouse splenocytes (naive or disease-specific) are activated under different conditions ex-vivo
and immunological effects evaluated. In this experimental setup, we activate inflammatory cells in 3D cultures, closely resembling the local inflammatory response in-vivo. The assay can be adapted to target cells and inflammatory markers of interest.
Mouse splenocytes are activated in a 3D structure with LPS, anti-CD3/anti-CD28, ConA or disease related antigen (depending on cell type and pathway of interest) in presence of reference drugs or test item for 24 hours and analysed for cellular marker expression using flow cytometry. The supernatant is analysed for cytokine profile using luminex technology. In additon to the standard readouts, there is an option to add on analysis of proliferation and
viability or other project specific functional assays.
CELLULAR COMPOSITION (FLOW CYTOMETRY)
Mouse splenocytes (from naive mice or mice induced with inflammatory disease) with anti-CD3/CD28 (T cells), LPS (innate immune system) or disease relevant antigen in presence of reference drug e.g. Dexamethasone and test item for 24 hours. Cells analysed for expression of cellular markers and cellular composition using flow cytometry.
CYTOKINE PROFILE (LUMINEX)
Supernatant is investigated for cytokine profile using Luminex and a standard inflammation panel or project specific custom adapted panel.
